Some X-ray Diffraction Patterns from Single Crystals of the Large Ribosomal Subunit from Bacillus stearothermophilus X-ray diffraction patterns of three-dimensional crystals of the large ribosomal subunit from Bacillus stearothemwphilus have been obtained using a synchrotron radiation
نویسنده
چکیده
X-ray diffraction patterns of three-dimensional crystals of the large ribosomal subunit from Bacillus stearothemwphilus have been obtained using a synchrotron radiation beam. The patterns contain resolved diffraction spots and indicate packing in relatively small unit cells, the dimensions of which have been tentatively determined. The internal order of the crystals, as reflected in these patterns, has been correlated with the size, shape and conditions of growth of these crystals.
منابع مشابه
Characterization of single crystals of the large ribosomal particles from Bacillus stearothermophilus.
Single, three-dimensional crystals of the 50 S ribosomal subunit from Bacillus stearothermophilus (strain NCA) have been characterized using a synchrotron X-ray source. The crystals are orthorhombic with unit cell dimensions: a = 350 A, b = 670 A, c = 905 A, and contain at least one 2-fold screw axis. With cooling to -2 degrees C, the large crystals (1.0 mm X 0.2 mm X 0.1 mm) diffract to 15 to ...
متن کاملApproaching the molecular structure of ribosomes.
Fifteen forms of three-dimensional crystals and three forms of two-dimensional sheets from ribosomal particles have been grown. In all cases only biologically active particles could be crystallized, the crystalline material retaining its integrity and biological activity for months. Cryastallographic data have been collected from crystals of 50 S ribosomal subunits, using synchrotron radiation,...
متن کاملTwo-dimensional crystalline sheets of Bacillus stearothermophilus 50S ribosomal particles.
Two-dimensional crystalline sheets of the large ribosomal subunit from Bacillus stearothermophilus have been obtained using a slightly modified procedure to that for growing three-dimensional crystals of the same material. The crystalline subunits are packed within monolayers in a relatively small unit cell, the dimensions of which are closely related to those observed for two forms of the thre...
متن کاملThe crystallization of ribosomal proteins from the 50 S subunit of the Escherichia coli and Bacillus stearothermophilus ribosome.
Several individual intact ribosomal proteins purified from bacterial sources under mild conditions have been crystallized. A number of these are suitable candidates for three-dimensional structural studies by x-ray diffraction techniques. Data collection to 3 A resolution for one of these proteins is in progress.
متن کاملGoniometer-based femtosecond X-ray diffraction of mutant 30S ribosomal subunit crystals
In this work, we collected radiation-damage-free data from a set of cryo-cooled crystals for a novel 30S ribosomal subunit mutant using goniometer-based femtosecond crystallography. Crystal quality assessment for these samples was conducted at the X-ray Pump Probe end-station of the Linac Coherent Light Source (LCLS) using recently introduced goniometer-based instrumentation. These 30S subunit ...
متن کامل